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KMID : 0358419960390081479
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Korean Journal of Obstetrics and Gynecology
1996 Volume.39 No. 8 p.1479 ~ p.1506
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The Effects of 17-βEtradiol, Medroxyprogesterone Acetate and Parathyroid Hormone on the Differentiation of Osteoblast Cell
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조수현
김광진/김승룡/이재억/문형/황윤영
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Abstract
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Since the effects of estrogen on osteoblast differentiation were not well known, the effects of estradiol on alkaline phosphatase activity were evaluated in rat osteosarcoma derived osteoblast-like cells(ROS-17/2.8-5) and human osteosarcoma
derived
osteoblast-like cells(OST). Both osteoblast cells were cultured in DMEM in the presence(+Dex) and absence(-Dex) of 10nM dexamethasone for days. The medium was then replaced with serum free medium containing 0.1% BSA with or without Dex, and
incubation
continued for a futher 48 hours. It showed that the +Dex cells had higher alkaline phosphatase(ALP) activity than the -Dex cells. 17β-estradiol had significant stimulatory effects on alkaline phosphatase activity in ROS-Dex cells, but inhibitory
effects on ROS+Dex cells, OST+Dex cells. Medroxyprogesterone acetate (MPA) had no effects on ALP activity in both ROS, OST cells. Parathyroid hormone(PTH) significantly inhibit the ALP activity in all cells except ROS+Dex cells. 17 β-estradiol
and
MPA
synergistically stimulate the ALP activity in OST+Dex cells. Synergism between 17β-estradiol and PTH have been observed in OST+Dex cells. 17β-estradiol blocked the effects of Dex and PTH while MPA did block the effects of PTH action on ALP
activity.
Although the effects of hormonal response of osteoblatic cells in cluture depended on species and stage of differentiation of the cells, it could be suggested that 17β-estradiol had direct effects on osteoblast differentiation.
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